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黄鳍鲷2个自然群体遗传变异的RAPD分析 总被引:6,自引:0,他引:6
用RAPD技术对福建和珠江口2个自然黄鳍鲷(Sparus latus)群体进行群体内与群体间的遗传变异分析。在使用的40个随机引物中.有18个引物扩增出清晰稳定的片段,共计93条,大小为200-2500bp,其中多各性片段41条.多态性片段的比例为44.09%。福建和珠江口群体内的相似系数分别为0.8821和0.8785.群体内遗传距离分别为0.1179和0.1215,2个群体间的遗传距离为0.1314。福建和珠江口2个群体内都有较高程度的遗传变异水平,但福建群体的遗传变异水平较珠江口群体的低。用类平均聚类法和邻接法进行聚类分析,结果表明.福建和珠江口群体分别聚成一支,且两者的聚类结果一致。说明福建和珠江口群体已经开始出现了遗传分化,分别属于2个不同的地理群体。 相似文献
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Nitrogen balance method and nitrogen-free diet were used in this study to determine nitrogen maintenance requirement (NM)
and nitrogen maintenance requirement per unit metabolism body weight (NM′) of black porgy Acanthopagrus schlegeli. Fish with body weight (BW) of 50, 80, 120, 160 and 200 g were fed by the diets containing three graded levels of crude protein
(380, 420 and 460 g/kg). The results from nitrogen balance experiment showed that the amount of nitrogen deposition varied
from 0.15 to 0.31 mg/g BW per day, accounting for 12.2% to 21.1% of nitrogen intake. The amount of fecal nitrogen excretion
varied from 0.21 to 0.32 mg/g BW per day, accounting for 16.3% to 21.6% of nitrogen intake. The endogenous nitrogen excretion,
a main part of nitrogen consumption varied from 0.79 to 0.97 mg/g BW per day, accounting for 63.3 % to 68.0% of nitrogen intake.
Positive correlation was found between NM and body weight, while a negative correlation was found between NM of unit body
weight and the growth duration. No significant differences (p>0.05) were found in NM′ among different growth stages. The average of NM′ was 0.485 7mg/g per day. The results from nitrogen-free
diet experiment showed that a negative correlation between NM and feed intake of nitrogen-free diet. NM increased with the
decrease of feed intake of fish. The average of NM was 0.482 9 mg/g BW per day that was close to 0.483 8 mg/g BW obtained
from fish with 120 g BW in nitrogen balance experiment. The nitrogen balance method is recommended to be a better method for
determining NM in consideration of fish stress and result stability. This study also provides a calculated result of the protein
content in diets, which is necessary for maintaining fish body protein at different growth stages. The calculation was based
on the amount of nitrogen required for maintaining body protein per kg BW.
Supported by Scientific Research Project Grant (No.2004C100059) from the city government of Ningbo, China. 相似文献
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鱼类Ghrelin的研究主要集中在摄食和生长方面,在免疫方面的研究甚少。本文以沿海经济鱼类黑鲷(Acanthopagrus schlegelii)为研究对象,检测了不同盐度(10、20、30)环境下腹腔注射Ghrelin(生理浓度,10ng/100g体重)对黑鲷部分免疫指标(超氧化物歧化酶SOD、溶菌酶活性LYS、血清补体3含量C3、免疫球蛋白M含量Ig M和丙二醛含量MDA)的影响。结果如下:在30盐度(正常盐度)时,腹腔注射Ghrelin对SOD和C3无显著影响(P0.05),1h后Ig M含量显著提高(P0.05),2h后LYS和MDA也明显增加(P0.05);在20盐度(轻盐度胁迫)时,腹腔注射Ghrelin不影响Ig M含量(P0.05),1h后SOD、LYS、C3均有明显上升(P0.05),而MDA则显著下降(P0.05),影响持续时间较长;在10盐度(重盐度胁迫)时,腹腔注射Ghrelin对所检测的5种免疫指标均有显著影响,其中1h后LYS、C3、Ig M含量明显提高(P0.05),3h后MDA下降,6h后SOD明显升高(P0.05)。以上结果表明:在低盐度肋迫下,Ghrelin可显著提高海水鱼黑鲷血清中SOD、LYS、C3和Ig M水平,降低MDA水平(P0.05),使黑鲷免疫力提高,以缓解其对低盐度胁迫的适应。因此,Ghrelin在海鱼的淡化驯养中具有潜在的应用前景,有望开发为一种海鱼淡化驯养的饲料添加剂。 相似文献
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为探明珠江口黑鲷海捕苗种与人工繁殖苗种间的形态差异,作者采用3种多元统计分析和方差分析方法,对采集自珠江口两岸的海捕黑鲷(Acanthopagrusschlegelii)苗种与2个繁殖场的人工繁殖苗种进行形态差异比较,比较了不同样品组的外部形态特征差异。可量性状主成分分析结果表明,前2项主成分的累计贡献率为89.90%,聚类分析结果表明汕头苗场(ST)样品组与另外3个样品组距离最远,判别分析结果同样显示以对该样品组判别准确率最高;可数性状单因素方差分析结果表明,样品组间背鳍鳍条数、鳃耙数、椎骨数存在显著性差异(P0.05)。主成分分析、聚类分析、判别分析和单因素方差分析从不同角度反映了样品组间的形态差异,各分析方法获得结论基本类似,相互印证。该研究结果显示,采集自不同繁殖场的黑鲷苗种与珠江口海域野生苗种存在一定的形态差异,但没有证据显示这种差异已导致黑鲷野生和养殖群体间明显的形态分化。 相似文献
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Nitrogen balance method and nitrogen-free diet were used in this study to determine nitrogen maintenance requirement (NM) and nitrogen maintenance requirement per unit metabolism body weight (NM’) of black porgy Acanthopagrus schlegeli. Fish with body weight (BW) of 50, 80, 120, 160 and 200 g were fed by the diets containing three graded levels of crude protein (380, 420 and 460 g/kg). The results from nitrogen balance experiment showed that the amount of nitrogen deposition varied from 0.15 to 0.31 mg/g BW... 相似文献
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五种海水鱼视网膜结构的比较 总被引:6,自引:0,他引:6
本文比较和分析了黄鳍鲷,普通鲻鱼,赤点石斑鱼,蓝圆Shen,金色小沙丁鱼的视网膜形态特点,生理机能及其与生活环境的关系。指出这五种鱼视膜均为混合型网膜。按主要形态特点划分,这五种鱼视网膜可归纳为三类。这五种鱼的视锥,视杆和色素上皮细胞黑色素均参与视网膜运动反应。这五种鱼视网膜上都存在着单锥和两个内段染色反应相同的孪生双锥。 相似文献
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黄鳍鲷不同组织同工酶的研究 总被引:13,自引:2,他引:13
运用聚丙烯酰胺垂直梯度凝胶电泳法对黄鳍鲷的7种组织(心肌、肝脏、肾脏、肌肉、性腺、脾脏、脑)进行了4种同工酶(EST,LDH,MDH,ME)的初步研究,并对几种酶的同工酶位点及酶谱表型进行了分析。结果表明:黄鳍鲷的4种同工酶系统具有明显的组织特异性。同时还分析了聚丙烯酰胺垂直梯度凝胶电泳法对同工酶电泳的分离效果,认为该种方法对同工酶系统的分辨率高,分离效果好,可得到更精确的结果。 相似文献